Sample Preparation for STR DNA Profiling - Cell Line Authentication Testing


Preparing your human cell line for authentication at Genetica DNA Laboratories is a simple process.  





Fresh or frozen cell pellet:  


Please provide between 100,000 and 5 million cells (note the total cell count on the Submission Form)
For fresh cells, wash the cells in buffer prior to shipping to remove any traces of media or trypsin and note the storage medium on the Submission Form (i.e. buffer, etc.). 
Frozen cell pellets can be shipped 'as is' but be sure to note the storage medium on the Submission Form (i.e. cryopreservative, etc.). 
Must be shipped on dry ice/ice packs overnight




Dried cell pellet:   


Please provide between 100,000 and 5 million cells (note the total cell count on the Submission Form)
Wash the cells in buffer prior to drying the pellet to remove any traces of media or trypsin
Please be sure that the pellet is completely dry (no residual liquid) using a vacuum system (speed drying only - no/low heat only)
Can be shipped at ambient temperature


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Genomic DNA:  We accept the DNA extract for testing at a reduced cost per sample.


Please put the DNA concentration of the sample on the Submission Form


Minimum volume: 20uL
Minimum concentration:  10ng/uL


A DNA:RNA ratio of around 1.7 provides the best results
Can be shipped at ambient temperatureType your paragraph here.

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